Proteomics - PAPPSO (GQE-Le Moulon)

Technologies

• Protein identification and quantification by mass spectrometry (LC-MS/MS)
• Identification and quantification of posttranslational modifications by mass spectrometry (LC-MS/MS)
• Development of bio-informatics tools and softwares for proteomics data analysis (peptide and protein identification, quantification, statistics)
• Trainings of users and collaborators in the use of the bioinformatics tools developed by PAPPSO

Equipment

Mass spectrometers (LC-MS / MS):

• Orbitrap Fusion Lumos tribrid (Thermo, acquired in December 2015, first installed in France), coupled to a nanoUPLC (Ultimate RSLCnano 3000, Thermo)
  > Characterization of multiple post-translational modifications

• Qexactive-Plus (Thermo), coupled to a nanoUPLC (nanoLC-Ultra 2D Eksigent)
  > Analysis of complex protein mixtures (identification and quantification) or determination of post-translational modifications

• LTQ Orbitrap, coupled to a nanoUPLC (Ultimate RSLCnano 3000, Thermo)
  > Analysis for identification and quantification of protein mixtures

• LTQ-XL, coupled to a nanoUPLC (EASY-nLC 1000, Thermo)
  > Identification on 2D spots or SDS-PAGE bands

Sample preparation:

• Digestion robot for in-gel hydrolysis of proteins (Progest, Genomic Solution)
• Two chromatographic systems for the separation of proteins / peptides before the LC-MS / MS analysis:
  - Low pressure system (FPLC ÄKTA Purifier, Amersham)
  - High Pressure System (HPLC Ultimate, Dionex)

National Strategic Platform