Abstract:
Sustainable agriculture and the use of plants for biomass and green chemistry will require biochemical plant engineering to re-channel plant metabolism. These objectives are impeded by our poor understanding of metabolic steps that control complex plant metabolic networks and the lack of an integrative, dynamic view of plant metabolism. It is therefore important to identify metabolic bottlenecks to guide plant breeding and to drive genetic engineering. A major aim of this project was to provide an integrated view of interactions between plant metabolic functions like photosynthesis, photorespiration, the Krebs cycle, N-assimilation, amino acid metabolism and NAD synthesis including post-translational regulatory mechanisms and metabolic fluxes. Complementary approaches including biochemistry, recombinant protein technologies, leaf gas exchange, 13C and 15N labelling, metabolomics, phosphoproteomics, and reverse genetics were used to achieve this. Major results include: The identification of light/dark differential phosphorylations of primary metabolism enzymes including photorespiratory proteins and enzymes involved in glycolytic and alternative serine biosynthesis pathways. Evidence showing NAD biosynthesis is a process that limits plant yield. A coordinated interplay between photosynthetic and photorespiratory activities is required for plant development in air since a blocked photorespiratory cycle negatively impacts photosynthesis thus altering C-allocation and RuBisCo amounts. The presence of an alternative pathway for respiratory C-delivery via lysine synthesis and recycling that contributes to metabolic flexibility. Prospects include carrying out translational biology to provide evidence that increasing NAD biosynthesis to improve yield is applicable to crop plants and the possibility to modulate protein phosphorylation to improve photosynthetic CO2 assimilation and yield.
Publications:
Abadie C., Boex-Fontvieille E.R., Carroll A.J., Tcherkez G. (2016). In vivo stoichiometry of photorespiratory metabolism. Nature Plants 2: doi:10.1038/nplants.2015.1220.
Abadie C., Mainguet S., Davanture M., Hodges M., Zivy M., Tcherkez G. (2016). Concerted Changes in the Phosphoproteome and Metabolome Under Different CO2/O-2 Gaseous Conditions in Arabidopsis Rosettes. Plant and Cell Physiology 57(7): 1544-1556.
Baslam M, Mitsui T, Hodges M, Priesack E, Herritt MT, Aranjuelo I, Sanz-Sáez A. (2020) Photosynthesis in a changing global climate: Scaling up and scaling down in crops. Front Plant Sci. 11:882
Boex-Fontvieille E., Daventure M., Jossier M., Zivy M., Hodges M., Tcherkez G. (2013). Photosynthetic Control of Arabidopsis Leaf Cytoplasmic Translation Initiation by Protein Phosphorylation. PLOS ONE 8(7): e70692.
Boex-Fontvieille E.R.A., Gauthier P.P.G., Gilard F., Hodges M., Tcherkez G.G.B. (2013). A new anaplerotic respiratory pathway involving lysine biosynthesis in isocitrate dehydrogenase-deficient Arabidopsis mutants. New Phytol 199(3): 673-682.
Boex-Fontvieille E., Davanture M., Jossier M., Zivy M., Hodges M., Tcherkez G. (2014). Photosynthetic activity influences cellulose biosynthesis and phosphorylation of proteins involved therein in Arabidopsis leaves. Journal of Experimental Botany 65(17): 4997-5010.
Boex-Fontvieille E., Daventure M., Jossier M., Hodges M., Zivy M., Tcherkez G. (2014). Phosphorylation pattern of Rubisco activase in Arabidopsis leaves. Plant Biology 16(3): 550-557.
Boex-Fontvieille E., Jossier M., Davanture M., Zivy M., Hodges M., Tcherkez G. (2014). Differential Protein Phosphorylation Regulates Chloroplast Movement in Response to Strong Light and Darkness in Arabidopsis thaliana. Plant Molecular Biology Reporter 32(5): 987-1001.
Dellero Y., Lamothe-Sibold M., Jossier M., Hodges M. (2015). Arabidopsis thaliana ggt1 photorespiratory mutants maintain leaf carbon/nitrogen balance by reducing RuBisCO content and plant growth. Plant Journal 83(6): 1005-1018.
Dellero Y., Mauve C., Boex-Fontvieille E., Flesch V., Jossier M., Tcherkez G., Hodges M. (2015). Experimental evidence for a hydride transfer mechanism in plant glycolate oxidase catalysis. Journal of Biological Chemistry 290(3): 1689-1698.
Dellero Y., Jossier M., Glab N., Oury C., Tcherkez G., Hodges M. (2016). Decreased glycolate oxidase activity leads to altered carbon allocation and leaf senescence after a transfer from high CO2 to ambient air in Arabidopsis thaliana. Journal of Experimental Botany 67(10): 3149-3163.
Dellero Y., Jossier M., Schmitz J., Maurino V.G., Hodges M. (2016). Photorespiratory glycolate-glyoxylate metabolism. Journal of Experimental Botany 67(10): 3041-3052.
Duminil P, Davanture M, Oury C, Boex-Fontvieille E, Tcherkez G, Zivy M, Hodges M, Glab M (2021) Arabidopsis thaliana 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 activity requires serine 82 phosphorylation. Plant J. 107(5): 1478-1489
Ghashghaie J., Tcherkez G. (2013). Chapter Eight - Isotope Ratio Mass Spectrometry Technique to Follow Plant Metabolism: Principles and Applications of 12C/13C Isotopes. In Advances in Botanical Research, R. Dominique, ed (Academic Press), pp. 377-405.
Hao J, Petriacq P, de Bont L, Hodges M, Gakiere B (2018) Characterization of l-aspartate oxidase from Arabidopsis thaliana. Plant Sci 271, 133–142.
Hodges M., Jossier M., Boex-Fontvieille E., Tcherkez G. (2013). Protein phosphorylation and photorespiration. Plant Biology 15(4): 694-706.
Hodges M., Dellero Y., Keech O., Betti M., Raghavendra A.S., Sage R., Zhu X.G., Allen D.K., Weber A.P.M. (2016). Perspectives for a better understanding of the metabolic integration of photorespiration within a complex plant primary metabolism network. Journal of Experimental Botany 67(10): 3015-3026.
Hodges M (2023) Photosynthesis and improving photosynthesis. Progress in Botany (eds. Cánovas FM, Lüttge U, Risueño M-C, Pretzsch H), Springer. p1-49
Liu Y., Mauve C., Lamothe-Sibold M., Guérard F., Glab N., Hodges M., Jossier M. (2019) Photorespiratory serine hydroxymethyltransferase 1 activity impacts abiotic stress tolerance and stomatal closure. Plant Cell Environ 42, 2567-2583.
Tcherkez G. (2013). Is the recovery of (photo) respiratory CO2 and intermediates minimal? New Phytol 198(2): 334-338.
Communications in conferences:
Gordon conference “CO2 assimilation in plants”, Waterville, USA (June 2014): “Interactions between day respiration and photorespiration”, Guillaume Tcherkez.
Photorespiration: Key to better crops, Warnemünde, Germany (June 2015): “Protein phosphorylation and photorespiration”, Michael Hodges
Journées de la Sociéte Française de Photosynthèse, ENS Paris, France (June 2016): “The interaction between photosynthesis and photorespiration in Arabidopsis leaves”, Michael Hodges
Gordon Research Conference : Adapting Plants to Insure Against an Uncertain Future: CO2 Assimilation in Plants from Genome to Biome, Lucca Italy (2017) Phosphoregulation of photorespiratory enzymes. Michael Hodges
2nd International SPS conference: Plant Sciences for the Future, Orsay France (July 2018) Making photosynthesis great again. Invitation SPS.
KAAB International Symposium: Frontiers in Plant Science and Biotechnology, Niigata Japan (2018) Trying to improve photosynthesis by modifying photorespiration and stomatal movements. Invitation M. Baslam.
Other outputs:
Patent: De Bont L., Gakière B. French Patent 14 51445 « Plantes à rendement accru et méthode d’obtention de telles plantes » (2014), extension (24 fev 2015) PCT WO2015124799 : EP3110832A1 (Europe), US20160362702 (USA)
